Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors. / Loeth, Nina; Assing, Kristian; Madsen, Hans O; Vindeløv, Lars; Buus, Soren; Buus, Anette Stryhn.

I: PloS one, Bind 7, Nr. 2, e31420, 07.02.2012.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Loeth, N, Assing, K, Madsen, HO, Vindeløv, L, Buus, S & Buus, AS 2012, 'Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors', PloS one, bind 7, nr. 2, e31420. https://doi.org/10.1371/journal.pone.0031420

APA

Loeth, N., Assing, K., Madsen, H. O., Vindeløv, L., Buus, S., & Buus, A. S. (2012). Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors. PloS one, 7(2), [e31420]. https://doi.org/10.1371/journal.pone.0031420

Vancouver

Loeth N, Assing K, Madsen HO, Vindeløv L, Buus S, Buus AS. Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors. PloS one. 2012 feb. 7;7(2). e31420. https://doi.org/10.1371/journal.pone.0031420

Author

Loeth, Nina ; Assing, Kristian ; Madsen, Hans O ; Vindeløv, Lars ; Buus, Soren ; Buus, Anette Stryhn. / Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors. I: PloS one. 2012 ; Bind 7, Nr. 2.

Bibtex

@article{77bea013dd2c4d33842385e4d2acd303,
title = "Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors",
abstract = "CMV status is an important risk factor in immune compromised patients. In hematopoeitic cell transplantations (HCT), both donor and recipient are tested routinely for CMV status by serological assays; however, one might argue that it might also be of relevance to examine CMV status by cellular (i.e., T lymphocyte) assays. Here, we have analyzed the CMV status of 100 healthy blood bank donors using both serology and cellular assays. About half (56%) were found to be CMV seropositive, and they all mounted strong CD8+ and/or moderate CD4+ T cell responses ex vivo against the immunodominant CMV protein, pp65. Of the 44 seronegative donors, only five (11%) mounted ex vivo T cell responses; surprisingly, 33 (75%) mounted strong CD4+ T cell responses after a brief in vitro peptide stimulation culture. This may have significant implications for the analysis and selection of HCT donors.",
keywords = "CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Cytomegalovirus, Cytomegalovirus Infections, Humans, Immunity, Cellular, Immunity, Humoral, Phosphoproteins, Tissue Donors, Viral Matrix Proteins",
author = "Nina Loeth and Kristian Assing and Madsen, {Hans O} and Lars Vindel{\o}v and Soren Buus and Buus, {Anette Stryhn}",
year = "2012",
month = feb,
day = "7",
doi = "10.1371/journal.pone.0031420",
language = "English",
volume = "7",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

RIS

TY - JOUR

T1 - Humoral and cellular CMV responses in healthy donors; identification of a frequent population of CMV-specific, CD4+ T cells in seronegative donors

AU - Loeth, Nina

AU - Assing, Kristian

AU - Madsen, Hans O

AU - Vindeløv, Lars

AU - Buus, Soren

AU - Buus, Anette Stryhn

PY - 2012/2/7

Y1 - 2012/2/7

N2 - CMV status is an important risk factor in immune compromised patients. In hematopoeitic cell transplantations (HCT), both donor and recipient are tested routinely for CMV status by serological assays; however, one might argue that it might also be of relevance to examine CMV status by cellular (i.e., T lymphocyte) assays. Here, we have analyzed the CMV status of 100 healthy blood bank donors using both serology and cellular assays. About half (56%) were found to be CMV seropositive, and they all mounted strong CD8+ and/or moderate CD4+ T cell responses ex vivo against the immunodominant CMV protein, pp65. Of the 44 seronegative donors, only five (11%) mounted ex vivo T cell responses; surprisingly, 33 (75%) mounted strong CD4+ T cell responses after a brief in vitro peptide stimulation culture. This may have significant implications for the analysis and selection of HCT donors.

AB - CMV status is an important risk factor in immune compromised patients. In hematopoeitic cell transplantations (HCT), both donor and recipient are tested routinely for CMV status by serological assays; however, one might argue that it might also be of relevance to examine CMV status by cellular (i.e., T lymphocyte) assays. Here, we have analyzed the CMV status of 100 healthy blood bank donors using both serology and cellular assays. About half (56%) were found to be CMV seropositive, and they all mounted strong CD8+ and/or moderate CD4+ T cell responses ex vivo against the immunodominant CMV protein, pp65. Of the 44 seronegative donors, only five (11%) mounted ex vivo T cell responses; surprisingly, 33 (75%) mounted strong CD4+ T cell responses after a brief in vitro peptide stimulation culture. This may have significant implications for the analysis and selection of HCT donors.

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Cytomegalovirus

KW - Cytomegalovirus Infections

KW - Humans

KW - Immunity, Cellular

KW - Immunity, Humoral

KW - Phosphoproteins

KW - Tissue Donors

KW - Viral Matrix Proteins

U2 - 10.1371/journal.pone.0031420

DO - 10.1371/journal.pone.0031420

M3 - Journal article

C2 - 22347475

VL - 7

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 2

M1 - e31420

ER -

ID: 49597418