TY - JOUR

T1 - Pediatric-type high-grade neuroepithelial tumors with CIC gene fusion share a common DNA methylation signature

AU - Sievers, Philipp

AU - Sill, Martin

AU - Schrimpf, Daniel

AU - Abdullaev, Zied

AU - Donson, Andrew M.

AU - Lake, Jessica A.

AU - Friedel, Dennis

AU - Scheie, David

AU - Tynninen, Olli

AU - Rauramaa, Tuomas

AU - Vepsäläinen, Kaisa L.

AU - Samuel, David

AU - Chapman, Rebecca

AU - Grundy, Richard G.

AU - Pajtler, Kristian W

AU - Tauziède-Espariat, Arnault

AU - Métais, Alice

AU - Varlet, Pascale

AU - Snuderl, Matija

AU - Jacques, Thomas S

AU - Aldape, Kenneth

AU - Reuss, David E.

AU - Korshunov, Andrey

AU - Wick, Wolfgang

AU - Pfister, Stefan M

AU - von Deimling, Andreas

AU - Sahm, Felix

AU - Jones, David T.W.

N1 - Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Pediatric neoplasms in the central nervous system (CNS) show extensive clinical and molecular heterogeneity and are fundamentally different from those occurring in adults. Molecular genetic testing contributes to accurate diagnosis and enables an optimal clinical management of affected children. Here, we investigated a rare, molecularly distinct type of pediatric high-grade neuroepithelial tumor (n = 18), that was identified through unsupervised visualization of genome-wide DNA methylation array data, together with copy number profiling, targeted next-generation DNA sequencing, and RNA transcriptome sequencing. DNA and/or RNA sequencing revealed recurrent fusions involving the capicua transcriptional repressor (CIC) gene in 10/10 tumor samples analyzed, with the most common fusion being CIC::LEUTX (n = 9). In addition, a CIC::NUTM1 fusion was detected in one of the tumors. Apart from the detected fusion events, no additional oncogenic alteration was identified in these tumors. The histopathological review demonstrated a morphologically heterogeneous group of high-grade neuroepithelial tumors with positive immunostaining for markers of glial differentiation in combination with weak and focal expression of synaptophysin, CD56 and CD99. All tumors were located in the supratentorial compartment, occurred during childhood (median age 8.5 years) and typically showed early relapses. In summary, we expand the spectrum of pediatric-type tumors of the CNS by reporting a previously uncharacterized group of rare high-grade neuroepithelial tumors that share a common DNA methylation signature and recurrent gene fusions involving the transcriptional repressor CIC. Downstream functional consequences of the fusion protein CIC::LEUTX and potential therapeutic implications need to be further investigated.

AB - Pediatric neoplasms in the central nervous system (CNS) show extensive clinical and molecular heterogeneity and are fundamentally different from those occurring in adults. Molecular genetic testing contributes to accurate diagnosis and enables an optimal clinical management of affected children. Here, we investigated a rare, molecularly distinct type of pediatric high-grade neuroepithelial tumor (n = 18), that was identified through unsupervised visualization of genome-wide DNA methylation array data, together with copy number profiling, targeted next-generation DNA sequencing, and RNA transcriptome sequencing. DNA and/or RNA sequencing revealed recurrent fusions involving the capicua transcriptional repressor (CIC) gene in 10/10 tumor samples analyzed, with the most common fusion being CIC::LEUTX (n = 9). In addition, a CIC::NUTM1 fusion was detected in one of the tumors. Apart from the detected fusion events, no additional oncogenic alteration was identified in these tumors. The histopathological review demonstrated a morphologically heterogeneous group of high-grade neuroepithelial tumors with positive immunostaining for markers of glial differentiation in combination with weak and focal expression of synaptophysin, CD56 and CD99. All tumors were located in the supratentorial compartment, occurred during childhood (median age 8.5 years) and typically showed early relapses. In summary, we expand the spectrum of pediatric-type tumors of the CNS by reporting a previously uncharacterized group of rare high-grade neuroepithelial tumors that share a common DNA methylation signature and recurrent gene fusions involving the transcriptional repressor CIC. Downstream functional consequences of the fusion protein CIC::LEUTX and potential therapeutic implications need to be further investigated.

U2 - 10.1038/s41698-023-00372-1

DO - 10.1038/s41698-023-00372-1

M3 - Journal article

C2 - 36964296

AN - SCOPUS:85150994846

VL - 7

JO - n p j Precision Oncology

JF - n p j Precision Oncology

SN - 2397-768X

M1 - 30

ER -