Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity
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Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity. / Verder, Henrik; Heiring, Christian; Clark, Howard; Sweet, David; Jessen, Torben E; Ebbesen, Finn; Björklund, Lars J; Andreasson, Bengt; Bender, Lars; Bertelsen, Aksel; Dahl, Marianne; Eschen, Christian; Fenger-Grøn, Jesper; Hoffmann, Stine F; Höskuldsson, Agnar; Bruusgaard-Mouritsen, Maria; Lundberg, Fredrik; Postle, Anthony D; Schousboe, Peter; Schmidt, Peter; Stanchev, Hristo; Sørensen, Lars.
I: Acta Paediatrica, Bind 106, Nr. 3, 2017, s. 430-437.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted respiratory distress syndrome with high sensitivity
AU - Verder, Henrik
AU - Heiring, Christian
AU - Clark, Howard
AU - Sweet, David
AU - Jessen, Torben E
AU - Ebbesen, Finn
AU - Björklund, Lars J
AU - Andreasson, Bengt
AU - Bender, Lars
AU - Bertelsen, Aksel
AU - Dahl, Marianne
AU - Eschen, Christian
AU - Fenger-Grøn, Jesper
AU - Hoffmann, Stine F
AU - Höskuldsson, Agnar
AU - Bruusgaard-Mouritsen, Maria
AU - Lundberg, Fredrik
AU - Postle, Anthony D
AU - Schousboe, Peter
AU - Schmidt, Peter
AU - Stanchev, Hristo
AU - Sørensen, Lars
N1 - ©2016 The Authors. Acta Paediatrica published by John Wiley & Sons Ltd on behalf of Foundation Acta Paediatrica.
PY - 2017
Y1 - 2017
N2 - AIM: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation.METHODS: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 μL of aspirate.RESULTS: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results.CONCLUSION: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.
AB - AIM: Respiratory distress syndrome (RDS) is a major cause of mortality and morbidity in premature infants. By the time symptoms appear, it may already be too late to prevent a severe course, with bronchopulmonary dysplasia or mortality. We aimed to develop a rapid test of lung maturity for targeting surfactant supplementation.METHODS: Concentrations of the most surface-active lung phospholipid dipalmitoylphosphatidylcholine and sphingomyelin in gastric aspirates from premature infants were measured by mass spectrometry and expressed as the lecithin/sphingomyelin ratio (L/S). The same aspirates were analysed with mid-infrared spectroscopy. Subsequently, L/S was measured in gastric aspirates and oropharyngeal secretions from another group of premature infants using spectroscopy and the results were compared with RDS development. The 10-minute analysis required 10 μL of aspirate.RESULTS: An L/S algorithm was developed based on 89 aspirates. Subsequently, gastric aspirates were sampled in 136 infants of 24-31 weeks of gestation and 61 (45%) developed RDS. The cut-off value of L/S was 2.2, sensitivity was 92%, and specificity was 73%. In 59 cases, the oropharyngeal secretions had less valid L/S than gastric aspirate results.CONCLUSION: Our rapid test for lung maturity, based on spectroscopy of gastric aspirate, predicted RDS with high sensitivity.
U2 - 10.1111/apa.13683
DO - 10.1111/apa.13683
M3 - Journal article
C2 - 27886403
VL - 106
SP - 430
EP - 437
JO - Acta Paediatrica
JF - Acta Paediatrica
SN - 0803-5253
IS - 3
ER -
ID: 194910968