Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
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Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis. / Franck, Kristina Træholt; Gumpert, Heidi; Olesen, Bente; Larsen, Anders Rhod; Petersen, Andreas; Bangsborg, Jette; Albertsen, Per; Westh, Henrik T.; Bartels, Mette Damkjær.
I: Frontiers in Microbiology, Bind 8, 173, 2017.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
AU - Franck, Kristina Træholt
AU - Gumpert, Heidi
AU - Olesen, Bente
AU - Larsen, Anders Rhod
AU - Petersen, Andreas
AU - Bangsborg, Jette
AU - Albertsen, Per
AU - Westh, Henrik T.
AU - Bartels, Mette Damkjær
PY - 2017
Y1 - 2017
N2 - Denmark is a low prevalence country with regard to methicillin resistant Staphylococcus aureus (MRSA). In 2008 and 2014, two neonatal wards in the Copenhagen area experienced outbreaks with a typical community acquired MRSA belonging to the same spa type and sequence type (t015:ST45) and both were PVL and ACME negative. In outbreak 1, the isolates harbored SCCmec IVa and in outbreak 2 SCCmec V. The clinical presentation differed between the two outbreaks, as none of five MRSA positive mothers in outbreak 1 had mastitis vs. five of six MRSA positive mothers in outbreak 2 (p < 0.02). To investigate if whole-genome sequencing could identify virulence genes associated with mastitis, t015:ST45 isolates from Denmark (N = 101) were whole-genome sequenced. Sequence analysis confirmed two separate outbreaks with no sign of sustained spread into the community. Analysis of the accessory genome between isolates from the two outbreaks revealed a S. aureus pathogenicity island containing enterotoxin C and enterotoxin-like L only in isolates from outbreak 2. Enterotoxin C and enterotoxin-like L carrying S. aureus are associated with bovine mastitis and our findings indicate that these may also be important virulence factors for human mastitis.
AB - Denmark is a low prevalence country with regard to methicillin resistant Staphylococcus aureus (MRSA). In 2008 and 2014, two neonatal wards in the Copenhagen area experienced outbreaks with a typical community acquired MRSA belonging to the same spa type and sequence type (t015:ST45) and both were PVL and ACME negative. In outbreak 1, the isolates harbored SCCmec IVa and in outbreak 2 SCCmec V. The clinical presentation differed between the two outbreaks, as none of five MRSA positive mothers in outbreak 1 had mastitis vs. five of six MRSA positive mothers in outbreak 2 (p < 0.02). To investigate if whole-genome sequencing could identify virulence genes associated with mastitis, t015:ST45 isolates from Denmark (N = 101) were whole-genome sequenced. Sequence analysis confirmed two separate outbreaks with no sign of sustained spread into the community. Analysis of the accessory genome between isolates from the two outbreaks revealed a S. aureus pathogenicity island containing enterotoxin C and enterotoxin-like L only in isolates from outbreak 2. Enterotoxin C and enterotoxin-like L carrying S. aureus are associated with bovine mastitis and our findings indicate that these may also be important virulence factors for human mastitis.
U2 - 10.3389/fmicb.2017.00173
DO - 10.3389/fmicb.2017.00173
M3 - Journal article
C2 - 28223977
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
M1 - 173
ER -
ID: 195290681