Ulcerative Colitis-associated E. coli pathobionts potentiate colitis in susceptible hosts
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- Ulcerative Colitis associated E coli pathobionts potentiate colitis in susceptible hosts
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Ulcerative colitis (UC) is a chronic inflammatory condition linked to intestinal microbial dysbiosis, including the expansion of E. coli strains related to extra-intestinal pathogenic E. coli. These “pathobionts” exhibit pathogenic properties, but their potential to promote UC is unclear due to the lack of relevant animal models. Here, we established a mouse model using a representative UC pathobiont strain (p19A), and mice lacking single immunoglobulin and toll-interleukin 1 receptor domain (SIGIRR), a deficiency increasing susceptibility to gut infections. Strain p19A was found to adhere to the cecal mucosa of Sigirr -/- mice, causing modest inflammation. Moreover, it dramatically worsened dextran sodium sulfate-induced colitis. This potentiation was attenuated using a p19A strain lacking α-hemolysin genes, or when we targeted pathobiont adherence using a p19A strain lacking the adhesin FimH, or following treatment with FimH antagonists. Thus, UC pathobionts adhere to the intestinal mucosa, and worsen the course of colitis in susceptible hosts.
Originalsprog | Engelsk |
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Artikelnummer | 1847976 |
Tidsskrift | Gut Microbes |
Vol/bind | 12 |
Udgave nummer | 1 |
Antal sider | 19 |
ISSN | 1949-0976 |
DOI | |
Status | Udgivet - 2020 |
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