Neutralizing antibody and CD8+ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2

Neutralizing antibody and CD8⁺ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Neutralizing antibody and CD8⁺ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2. / Underwood, Alexander P.; Sølund, Christina; Jacobsen, Kivin; Binderup, Alekxander; Fernandez-Antunez, Carlota; Mikkelsen, Lotte S.; Inekci, Dilek; Villadsen, Signe Lysemose; Castruita, Jose A.S.; Pinholt, Mette; Fahnøe, Ulrik; Ramirez, Santseharay; Brix, Liselotte; Weis, Nina; Bukh, Jens.

I: Frontiers in Immunology, Bind 15, 1353353, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Underwood, AP, Sølund, C, Jacobsen, K, Binderup, A, Fernandez-Antunez, C, Mikkelsen, LS, Inekci, D, Villadsen, SL, Castruita, JAS, Pinholt, M, Fahnøe, U, Ramirez, S, Brix, L, Weis, N & Bukh, J 2024, 'Neutralizing antibody and CD8⁺ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2', Frontiers in Immunology, bind 15, 1353353. https://doi.org/10.3389/fimmu.2024.1353353

APA

Underwood, A. P., Sølund, C., Jacobsen, K., Binderup, A., Fernandez-Antunez, C., Mikkelsen, L. S., Inekci, D., Villadsen, S. L., Castruita, J. A. S., Pinholt, M., Fahnøe, U., Ramirez, S., Brix, L., Weis, N., & Bukh, J. (2024). Neutralizing antibody and CD8⁺ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2. Frontiers in Immunology, 15, [1353353]. https://doi.org/10.3389/fimmu.2024.1353353

Vancouver

Underwood AP, Sølund C, Jacobsen K, Binderup A, Fernandez-Antunez C, Mikkelsen LS o.a. Neutralizing antibody and CD8⁺ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2. Frontiers in Immunology. 2024;15. 1353353. https://doi.org/10.3389/fimmu.2024.1353353

Author

Underwood, Alexander P. ; Sølund, Christina ; Jacobsen, Kivin ; Binderup, Alekxander ; Fernandez-Antunez, Carlota ; Mikkelsen, Lotte S. ; Inekci, Dilek ; Villadsen, Signe Lysemose ; Castruita, Jose A.S. ; Pinholt, Mette ; Fahnøe, Ulrik ; Ramirez, Santseharay ; Brix, Liselotte ; Weis, Nina ; Bukh, Jens. / Neutralizing antibody and CD8⁺ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2. I: Frontiers in Immunology. 2024 ; Bind 15.

Bibtex

@article{fc685240ad2f4f5dab408308ac845c4f,

title = "Neutralizing antibody and CD8+ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2",

abstract = "As severe acute respiratory coronavirus 2 (SARS-CoV-2) variants continue to emerge, it is important to characterize immune responses against variants which can inform on protection efficacies following booster vaccination. In this study, neutralizing breadth and antigen-specific CD8+ T cell responses were analyzed in both infection-na{\"i}ve and infection-experienced individuals following administration of a booster bivalent Wuhan-Hu-1+BA.4/5 Comirnaty{\textregistered} mRNA vaccine. Significantly higher neutralizing titers were found after this vaccination compared to the pre-third booster vaccination time point. Further, neutralizing breadth to omicron variants, including BA.1, BA.2, BA.5, BQ.1 and XBB.1, was found to be boosted following bivalent vaccination. SARS-CoV-2-specific CD8+ T cells were identified, but with no evidence that frequencies were increased following booster vaccinations. Spike protein-specific CD8+ T cells were the only responses detected after vaccination and non-spike-specific CD8+ T cells were only detected after infection. Both spike-specific and non-spike-specific CD8+ T cells were found at much lower frequencies than CD8+ T cells specific to cytomegalovirus (CMV), Epstein-Barr virus (EBV) and influenza (Flu). Taken together, these results show that the bivalent Wuhan-Hu-1+BA.4/5 Comirnaty{\textregistered} mRNA vaccine boosted the breadth of neutralization to newer SARS-CoV-2 variants and that vaccination is able to induce spike protein-specific CD8+ T cell responses, which are maintained longitudinally.",

keywords = "CD8 T cells, COVID-19, neutralization, SARS-CoV-2, vaccination",

author = "Underwood, {Alexander P.} and Christina S{\o}lund and Kivin Jacobsen and Alekxander Binderup and Carlota Fernandez-Antunez and Mikkelsen, {Lotte S.} and Dilek Inekci and Villadsen, {Signe Lysemose} and Castruita, {Jose A.S.} and Mette Pinholt and Ulrik Fahn{\o}e and Santseharay Ramirez and Liselotte Brix and Nina Weis and Jens Bukh",

note = "Publisher Copyright: Copyright {\textcopyright} 2024 Underwood, S{\o}lund, Jacobsen, Binderup, Fernandez-Antunez, Mikkelsen, Inekci, Villadsen, Castruita, Pinholt, Fahn{\o}e, Ramirez, Brix, Weis and Bukh.",

year = "2024",

doi = "10.3389/fimmu.2024.1353353",

language = "English",

volume = "15",

journal = "Frontiers in Immunology",

issn = "1664-3224",

publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Neutralizing antibody and CD8+ T cell responses following BA.4/5 bivalent COVID-19 booster vaccination in adults with and without prior exposure to SARS-CoV-2

AU - Underwood, Alexander P.

AU - Sølund, Christina

AU - Jacobsen, Kivin

AU - Binderup, Alekxander

AU - Fernandez-Antunez, Carlota

AU - Mikkelsen, Lotte S.

AU - Inekci, Dilek

AU - Villadsen, Signe Lysemose

AU - Castruita, Jose A.S.

AU - Pinholt, Mette

AU - Fahnøe, Ulrik

AU - Ramirez, Santseharay

AU - Brix, Liselotte

AU - Weis, Nina

AU - Bukh, Jens

PY - 2024

Y1 - 2024

N2 - As severe acute respiratory coronavirus 2 (SARS-CoV-2) variants continue to emerge, it is important to characterize immune responses against variants which can inform on protection efficacies following booster vaccination. In this study, neutralizing breadth and antigen-specific CD8+ T cell responses were analyzed in both infection-naïve and infection-experienced individuals following administration of a booster bivalent Wuhan-Hu-1+BA.4/5 Comirnaty® mRNA vaccine. Significantly higher neutralizing titers were found after this vaccination compared to the pre-third booster vaccination time point. Further, neutralizing breadth to omicron variants, including BA.1, BA.2, BA.5, BQ.1 and XBB.1, was found to be boosted following bivalent vaccination. SARS-CoV-2-specific CD8+ T cells were identified, but with no evidence that frequencies were increased following booster vaccinations. Spike protein-specific CD8+ T cells were the only responses detected after vaccination and non-spike-specific CD8+ T cells were only detected after infection. Both spike-specific and non-spike-specific CD8+ T cells were found at much lower frequencies than CD8+ T cells specific to cytomegalovirus (CMV), Epstein-Barr virus (EBV) and influenza (Flu). Taken together, these results show that the bivalent Wuhan-Hu-1+BA.4/5 Comirnaty® mRNA vaccine boosted the breadth of neutralization to newer SARS-CoV-2 variants and that vaccination is able to induce spike protein-specific CD8+ T cell responses, which are maintained longitudinally.

AB - As severe acute respiratory coronavirus 2 (SARS-CoV-2) variants continue to emerge, it is important to characterize immune responses against variants which can inform on protection efficacies following booster vaccination. In this study, neutralizing breadth and antigen-specific CD8+ T cell responses were analyzed in both infection-naïve and infection-experienced individuals following administration of a booster bivalent Wuhan-Hu-1+BA.4/5 Comirnaty® mRNA vaccine. Significantly higher neutralizing titers were found after this vaccination compared to the pre-third booster vaccination time point. Further, neutralizing breadth to omicron variants, including BA.1, BA.2, BA.5, BQ.1 and XBB.1, was found to be boosted following bivalent vaccination. SARS-CoV-2-specific CD8+ T cells were identified, but with no evidence that frequencies were increased following booster vaccinations. Spike protein-specific CD8+ T cells were the only responses detected after vaccination and non-spike-specific CD8+ T cells were only detected after infection. Both spike-specific and non-spike-specific CD8+ T cells were found at much lower frequencies than CD8+ T cells specific to cytomegalovirus (CMV), Epstein-Barr virus (EBV) and influenza (Flu). Taken together, these results show that the bivalent Wuhan-Hu-1+BA.4/5 Comirnaty® mRNA vaccine boosted the breadth of neutralization to newer SARS-CoV-2 variants and that vaccination is able to induce spike protein-specific CD8+ T cell responses, which are maintained longitudinally.

KW - CD8 T cells

KW - COVID-19

KW - neutralization

KW - SARS-CoV-2

KW - vaccination

U2 - 10.3389/fimmu.2024.1353353

DO - 10.3389/fimmu.2024.1353353

M3 - Journal article

C2 - 38571939

AN - SCOPUS:85189149122

VL - 15

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

M1 - 1353353

ER -

ID: 388044068

Institut for Klinisk Medicin