TY - JOUR

T1 - Major contribution from recurrent alterations and MSH6 mutations in the Danish Lynch syndrome population.

AU - Nilbert, Mef

AU - Wikman, Friedrik P

AU - Hansen, Thomas V O

AU - Krarup, Henrik B

AU - Orntoft, Torben F

AU - Nielsen, Finn C

AU - Sunde, Lone

AU - Gerdes, Anne-Marie

AU - Cruger, Dorthe

AU - Timshel, Susanne

AU - Bisgaard, Søs Marie Luise

AU - Bernstein, Inge

AU - Okkels, Henrik

AU - Nilbert, Mef

AU - Wikman, Friedrik

AU - Hansen, Thomas

AU - Krarup, Henrik

AU - Orntoft, Torben

AU - Nielsen, Finn

AU - Sunde, Lone

AU - Gerdes, Anne-Marie

AU - Cruger, Dorthe

AU - Timshel, Susanne

AU - Bisgaard, Marie-Louise

AU - Bernstein, Inge

AU - Okkels, Henrik

N1 - Keywords: Adaptor Proteins, Signal Transducing; Colorectal Neoplasms, Hereditary Nonpolyposis; DNA Mutational Analysis; DNA-Binding Proteins; Denmark; Female; Founder Effect; Humans; Male; Middle Aged; MutS Homolog 2 Protein; Mutation; Nuclear Proteins

PY - 2009

Y1 - 2009

N2 - An increasing number of mismatch-repair (MMR) gene mutations have been identified in hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch syndrome. This study presents the population-based Danish MMR gene mutation profile, which contains 138 different MMR gene alterations. Among these, 88 mutations in 164 families are considered pathogenic and an additional 50 variants from 76 families are considered to represent variants of unknown pathogenicity. The different MMR genes contribute to 40% (MSH2), 29% (MLH1), and 22% (MSH6) of the mutations and the Danish population thus shows a considerably higher frequency of MSH6 mutations than previously described. Although 69/88 (78%) pathogenic mutations were present in a single family, previously recognized recurrent/founder mutations were causative in 75/137 (55%) MLH1/MSH2 mutant families. In addition, the Danish MLH1 founder mutation c.1667+2_1667_+8TAAATCAdelinsATTT was identified in 14/58 (24%) MLH1 mutant families. The Danish Lynch syndrome population thus demonstrates that MSH6 mutations and recurrent/founder mutations have a larger contribution than previously recognized, which implies that the MSH6 gene should be included in routine diagnostics and suggests that directed analysis of recurrent/founder mutations may be feasible e.g. in families were diagnostic material is restricted to archival tissue.

AB - An increasing number of mismatch-repair (MMR) gene mutations have been identified in hereditary nonpolyposis colorectal cancer (HNPCC) or Lynch syndrome. This study presents the population-based Danish MMR gene mutation profile, which contains 138 different MMR gene alterations. Among these, 88 mutations in 164 families are considered pathogenic and an additional 50 variants from 76 families are considered to represent variants of unknown pathogenicity. The different MMR genes contribute to 40% (MSH2), 29% (MLH1), and 22% (MSH6) of the mutations and the Danish population thus shows a considerably higher frequency of MSH6 mutations than previously described. Although 69/88 (78%) pathogenic mutations were present in a single family, previously recognized recurrent/founder mutations were causative in 75/137 (55%) MLH1/MSH2 mutant families. In addition, the Danish MLH1 founder mutation c.1667+2_1667_+8TAAATCAdelinsATTT was identified in 14/58 (24%) MLH1 mutant families. The Danish Lynch syndrome population thus demonstrates that MSH6 mutations and recurrent/founder mutations have a larger contribution than previously recognized, which implies that the MSH6 gene should be included in routine diagnostics and suggests that directed analysis of recurrent/founder mutations may be feasible e.g. in families were diagnostic material is restricted to archival tissue.

U2 - 10.1007/s10689-008-9199-3

DO - 10.1007/s10689-008-9199-3

M3 - Journal article

VL - 8

SP - 75

EP - 83

JO - Familial Cancer

JF - Familial Cancer

SN - 1389-9600

IS - 1

ER -