Quantification of 8-oxo-7,8-dihydro-2′-deoxyguanosine and 8-oxo-7,8-dihydro-guanosine concentrations in urine and plasma for estimating 24-h urinary output
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Among markers for oxidative stress urinary excretion 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydro-guanosine (8-oxoGuo) have been widely used in controlled and epidemiological studies, and are considered to represent intracellular markers of oxidation of DNA and RNA in the entire organism, respectively. Although being non-invasive, urinary methods have shortcomings. There is no established method for analysis of 8-oxodGuo and 8-oxoGuo in plasma and the few plasma values presented in the literature vary greatly. We here present a liquid chromatography mass spectrometry method with full validation for analysis of 8-oxodGuo and 8-oxoGuo in plasma. Further, we investigated the basis for our previously physiological model and show that a single plasma sample can be used to estimate the 24-h production of 8-oxoGuo, whereas we challenge the use of urinary 8-oxodGuo/creatinine ratio or plasma 8-oxodGuo as measures of oxidative stress.
Originalsprog | Engelsk |
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Tidsskrift | Free Radical Biology and Medicine |
Vol/bind | 172 |
Sider (fra-til) | 350-357 |
Antal sider | 8 |
ISSN | 0891-5849 |
DOI | |
Status | Udgivet - 2021 |
Bibliografisk note
Funding Information:
Katja Luntang Christensen is acknowledged for her help with preparation and analysis of samples.
Publisher Copyright:
© 2021 Elsevier Inc.
ID: 273643601