Pro-Inflammatory Cytokines Promote the Transcription of Circular RNAs in Human Pancreatic β Cells
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Pro-Inflammatory Cytokines Promote the Transcription of Circular RNAs in Human Pancreatic β Cells. / Kaur, Simranjeet; Frørup, Caroline; Mirza, Aashiq H.; Fløyel, Tina; Yarani, Reza; Colli, Maikel L.; Johannesen, Jesper; Størling, Joachim; Eizirik, Decio L.; Pociot, Flemming.
I: Non-coding RNA, Bind 8, Nr. 5, 69, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Pro-Inflammatory Cytokines Promote the Transcription of Circular RNAs in Human Pancreatic β Cells
AU - Kaur, Simranjeet
AU - Frørup, Caroline
AU - Mirza, Aashiq H.
AU - Fløyel, Tina
AU - Yarani, Reza
AU - Colli, Maikel L.
AU - Johannesen, Jesper
AU - Størling, Joachim
AU - Eizirik, Decio L.
AU - Pociot, Flemming
N1 - Publisher Copyright: © 2022 by the authors.
PY - 2022
Y1 - 2022
N2 - Circular RNAs (circRNAs) have recently been implicated in impaired β-cell function in diabetes. Using microarray-based profiling of circRNAs in human EndoC-βH1 cells treated with pro-inflammatory cytokines, this study aimed to investigate the expression and possible regulatory roles of circRNAs in human β cells. We identified ~5000 β-cell-expressed circRNAs, of which 84 were differentially expressed (DE) after cytokine exposure. Pathway analysis of the host genes of the DE circRNAs revealed the enrichment of cytokine signaling pathways, indicative of circRNA transcription from inflammatory genes in response to cytokines. Multiple binding sites for β-cell-enriched microRNAs and RNA-binding proteins were observed for the highly upregulated circRNAs, supporting their function as ‘sponges’ or ‘decoys’. We also present evidence for circRNA sequence conservation in multiple species, the presence of cytokine-induced regulatory elements, and putative protein-coding potential for the DE circRNAs. This study highlights the complex regulatory potential of circRNAs, which may play a crucial role during immune-mediated β-cell destruction in type 1 diabetes.
AB - Circular RNAs (circRNAs) have recently been implicated in impaired β-cell function in diabetes. Using microarray-based profiling of circRNAs in human EndoC-βH1 cells treated with pro-inflammatory cytokines, this study aimed to investigate the expression and possible regulatory roles of circRNAs in human β cells. We identified ~5000 β-cell-expressed circRNAs, of which 84 were differentially expressed (DE) after cytokine exposure. Pathway analysis of the host genes of the DE circRNAs revealed the enrichment of cytokine signaling pathways, indicative of circRNA transcription from inflammatory genes in response to cytokines. Multiple binding sites for β-cell-enriched microRNAs and RNA-binding proteins were observed for the highly upregulated circRNAs, supporting their function as ‘sponges’ or ‘decoys’. We also present evidence for circRNA sequence conservation in multiple species, the presence of cytokine-induced regulatory elements, and putative protein-coding potential for the DE circRNAs. This study highlights the complex regulatory potential of circRNAs, which may play a crucial role during immune-mediated β-cell destruction in type 1 diabetes.
KW - circRNA
KW - CRIM1
KW - EPSTI1
KW - human islets
KW - inflammation
KW - microarray
KW - miRNA
KW - non-coding RNA
KW - type 1 diabetes
KW - WARS
UR - http://www.scopus.com/inward/record.url?scp=85140612726&partnerID=8YFLogxK
U2 - 10.3390/ncrna8050069
DO - 10.3390/ncrna8050069
M3 - Journal article
C2 - 36287121
AN - SCOPUS:85140612726
VL - 8
JO - Non-coding RNA
JF - Non-coding RNA
SN - 2311-553X
IS - 5
M1 - 69
ER -
ID: 324558884