Microvascular damage secondary to thromboembologenic arterial injury--a new experimental model
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Microvascular damage secondary to thromboembologenic arterial injury--a new experimental model. / Thorwest, M; Fuglsang, J; Poulsen, J K; Toft, G; Hjortdal, V E.
I: Annales Chirurgiae et Gynaecologiae. Supplementum, Bind 87, Nr. 3, 1998, s. 213-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Microvascular damage secondary to thromboembologenic arterial injury--a new experimental model
AU - Thorwest, M
AU - Fuglsang, J
AU - Poulsen, J K
AU - Toft, G
AU - Hjortdal, V E
PY - 1998
Y1 - 1998
N2 - BACKGROUND AND AIMS: The isolated rat cremaster model is used extensively for evaluating the microcirculation secondary to arterial injury. Current techniques, however, do not allow for assessment of injury and effect within the same animal. The purpose of this study was to develop a model incorporating the following points: visualization of the upstream arterial injury and the downstream microvascular damage in the same animal, analysis of capillary density by randomization of measuring windows throughout the cremaster muscle, and simplification of the arterial injury.MATERIALS AND METHODS: Thirteen male Wistar rats were randomized into two groups. In group I, the entire isolation of the cremaster muscle was performed, without arterial damage. In group II, arterial damage consisting of a standardized pinch was applied to the feeding vessel.RESULTS AND CONCLUSIONS: It was possible to produce a simplified arterial injury and visualize the resulting downstream microvascular damage in the same animal, in a quantitative and randomized fashion. In group I, no thrombus formation was seen. In group II, all animals produced an embolizing arterial thrombus, which was dynamic within the first hour of observation. Capillary density was reduced from 6.5 to 3.5 capillaries/measuring window within the first hour after arterial thromboembolism.
AB - BACKGROUND AND AIMS: The isolated rat cremaster model is used extensively for evaluating the microcirculation secondary to arterial injury. Current techniques, however, do not allow for assessment of injury and effect within the same animal. The purpose of this study was to develop a model incorporating the following points: visualization of the upstream arterial injury and the downstream microvascular damage in the same animal, analysis of capillary density by randomization of measuring windows throughout the cremaster muscle, and simplification of the arterial injury.MATERIALS AND METHODS: Thirteen male Wistar rats were randomized into two groups. In group I, the entire isolation of the cremaster muscle was performed, without arterial damage. In group II, arterial damage consisting of a standardized pinch was applied to the feeding vessel.RESULTS AND CONCLUSIONS: It was possible to produce a simplified arterial injury and visualize the resulting downstream microvascular damage in the same animal, in a quantitative and randomized fashion. In group I, no thrombus formation was seen. In group II, all animals produced an embolizing arterial thrombus, which was dynamic within the first hour of observation. Capillary density was reduced from 6.5 to 3.5 capillaries/measuring window within the first hour after arterial thromboembolism.
KW - Animals
KW - Arteries/injuries
KW - Capillaries/injuries
KW - Disease Models, Animal
KW - Hemodynamics
KW - Male
KW - Microcirculation
KW - Muscle, Skeletal/blood supply
KW - Random Allocation
KW - Rats
KW - Rats, Wistar
KW - Reproducibility of Results
KW - Thromboembolism/complications
M3 - Journal article
C2 - 9825066
VL - 87
SP - 213
EP - 218
JO - Annales Chirurgiae et Gynaecologiae
JF - Annales Chirurgiae et Gynaecologiae
SN - 0355-9874
IS - 3
ER -
ID: 243521535