Postprandial gallbladder emptying in patients with type 2 diabetes: potential implications for bile-induced secretion of glucagon-like peptide 1
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Postprandial gallbladder emptying in patients with type 2 diabetes : potential implications for bile-induced secretion of glucagon-like peptide 1. / Sonne, David P; Rehfeld, Jens F; Holst, Jens Juul; Vilsbøll, Tina; Knop, Filip K.
I: European Journal of Endocrinology, Bind 171, Nr. 4, 10.2014, s. 407-419.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Postprandial gallbladder emptying in patients with type 2 diabetes
T2 - potential implications for bile-induced secretion of glucagon-like peptide 1
AU - Sonne, David P
AU - Rehfeld, Jens F
AU - Holst, Jens Juul
AU - Vilsbøll, Tina
AU - Knop, Filip K
N1 - © 2014 European Society of Endocrinology.
PY - 2014/10
Y1 - 2014/10
N2 - OBJECTIVE: Recent preclinical work has suggested that postprandial flow of bile acids into the small intestine potentiates nutrient-induced glucagon-like peptide 1 (GLP1(GCG)) secretion via bile acid-induced activation of the G protein-coupled receptor TGR5 in intestinal L cells. The notion of bile-induced GLP1 secretion combined with the findings of reduced postprandial gallbladder emptying in patients with type 2 diabetes (T2DM) led us to speculate whether reduced postprandial GLP1 responses in some patients with T2DM arise as a consequence of diabetic gallbladder dysmotility.DESIGN AND METHODS: In a randomised design, 15 patients with long-standing T2DM and 15 healthy age-, gender- and BMI-matched control subjects were studied during 75-g oral glucose tolerance test (OGTT) and three isocaloric (500 kcal) and isovolaemic (350 ml) liquid meals: i) 2.5 g fat, 107 g carbohydrate and 13 g protein; ii) 10 g fat, 93 g carbohydrate and 11 g protein; and iii) 40 g fat, 32 g carbohydrate and 3 g protein. Basal and postprandial plasma concentrations of glucose, insulin, C-peptide, glucagon, GLP1, glucose-dependent insulinotropic polypeptide (GIP), cholecystokinin and gastrin were measured. Furthermore, gallbladder emptying and gastric emptying were examined.RESULTS: Gallbladder emptying increased with increasing meal fat content, but no intergroup differences were demonstrated. GIP and GLP1 responses were comparable among the groups with GIP levels being higher following high-fat meals, whereas GLP1 secretion was similar after both OGTT and meals.CONCLUSIONS: In conclusion, patients with T2DM exhibited normal gallbladder emptying to meals with a wide range of fat content. Incretin responses were similar to that in controls, and an association with postprandial gallbladder contraction could not be demonstrated.
AB - OBJECTIVE: Recent preclinical work has suggested that postprandial flow of bile acids into the small intestine potentiates nutrient-induced glucagon-like peptide 1 (GLP1(GCG)) secretion via bile acid-induced activation of the G protein-coupled receptor TGR5 in intestinal L cells. The notion of bile-induced GLP1 secretion combined with the findings of reduced postprandial gallbladder emptying in patients with type 2 diabetes (T2DM) led us to speculate whether reduced postprandial GLP1 responses in some patients with T2DM arise as a consequence of diabetic gallbladder dysmotility.DESIGN AND METHODS: In a randomised design, 15 patients with long-standing T2DM and 15 healthy age-, gender- and BMI-matched control subjects were studied during 75-g oral glucose tolerance test (OGTT) and three isocaloric (500 kcal) and isovolaemic (350 ml) liquid meals: i) 2.5 g fat, 107 g carbohydrate and 13 g protein; ii) 10 g fat, 93 g carbohydrate and 11 g protein; and iii) 40 g fat, 32 g carbohydrate and 3 g protein. Basal and postprandial plasma concentrations of glucose, insulin, C-peptide, glucagon, GLP1, glucose-dependent insulinotropic polypeptide (GIP), cholecystokinin and gastrin were measured. Furthermore, gallbladder emptying and gastric emptying were examined.RESULTS: Gallbladder emptying increased with increasing meal fat content, but no intergroup differences were demonstrated. GIP and GLP1 responses were comparable among the groups with GIP levels being higher following high-fat meals, whereas GLP1 secretion was similar after both OGTT and meals.CONCLUSIONS: In conclusion, patients with T2DM exhibited normal gallbladder emptying to meals with a wide range of fat content. Incretin responses were similar to that in controls, and an association with postprandial gallbladder contraction could not be demonstrated.
KW - Adult
KW - Aged
KW - Area Under Curve
KW - Bile
KW - Biological Markers
KW - Blood Glucose
KW - C-Peptide
KW - Case-Control Studies
KW - Cholecystokinin
KW - Denmark
KW - Diabetes Mellitus, Type 2
KW - Dietary Fats
KW - Female
KW - Gallbladder Emptying
KW - Gastric Emptying
KW - Gastric Inhibitory Polypeptide
KW - Gastrins
KW - Glucagon
KW - Glucagon-Like Peptide 1
KW - Glucose Tolerance Test
KW - Humans
KW - Insulin
KW - Male
KW - Middle Aged
KW - Postprandial Period
KW - Receptors, G-Protein-Coupled
U2 - 10.1530/EJE-14-0309
DO - 10.1530/EJE-14-0309
M3 - Journal article
C2 - 24986531
VL - 171
SP - 407
EP - 419
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 4
ER -
ID: 132002600