CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T)

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Standard

CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T). / Larsen, Sylvester; Seidelin, Jakob Benedict; Davidsen, Johanne; Dahlgaard, Katja; Nielsen, Claus Henrik; Bennett, Eric Paul; Pedersen, Ole Birger; Coskun, Mehmet; Troelsen, Jesper Thorvald.

I: FEBS Open Bio, Bind 11, Nr. 6, 2021, s. 1638-1644.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Larsen, S, Seidelin, JB, Davidsen, J, Dahlgaard, K, Nielsen, CH, Bennett, EP, Pedersen, OB, Coskun, M & Troelsen, JT 2021, 'CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T)', FEBS Open Bio, bind 11, nr. 6, s. 1638-1644. https://doi.org/10.1002/2211-5463.13161

APA

Larsen, S., Seidelin, J. B., Davidsen, J., Dahlgaard, K., Nielsen, C. H., Bennett, E. P., Pedersen, O. B., Coskun, M., & Troelsen, J. T. (2021). CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T). FEBS Open Bio, 11(6), 1638-1644. https://doi.org/10.1002/2211-5463.13161

Vancouver

Larsen S, Seidelin JB, Davidsen J, Dahlgaard K, Nielsen CH, Bennett EP o.a. CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T). FEBS Open Bio. 2021;11(6):1638-1644. https://doi.org/10.1002/2211-5463.13161

Author

Larsen, Sylvester ; Seidelin, Jakob Benedict ; Davidsen, Johanne ; Dahlgaard, Katja ; Nielsen, Claus Henrik ; Bennett, Eric Paul ; Pedersen, Ole Birger ; Coskun, Mehmet ; Troelsen, Jesper Thorvald. / CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T). I: FEBS Open Bio. 2021 ; Bind 11, Nr. 6. s. 1638-1644.

Bibtex

@article{c3ab6e3ac7654d4f80cad1dbe9458023,
title = "CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T)",
abstract = "Dysregulation of interleukin 33 (IL-33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the intestinal-specific transcription factor CDX2 is a key component. In this study, we investigated whether CDX2 regulates IL33 mRNA expression. We examined IL33 mRNA expression in primary colonic epithelial cells from healthy humans and epithelial cell lines, revealing high expression levels in primary colonic and LS174T cells. Combining genomics data (ChIP-Seq, RNA-Seq) and IL33 promoter analyses in LS174T cells revealed intronic enhancer activity in the IL33 gene that is dependent on CDX2 expression. Western blotting and qRT-PCR confirmed that IL33 expression is upregulated in a CDX2 concentration-dependent manner, thereby providing the first evidence that CDX2 regulates the expression of IL33.",
author = "Sylvester Larsen and Seidelin, {Jakob Benedict} and Johanne Davidsen and Katja Dahlgaard and Nielsen, {Claus Henrik} and Bennett, {Eric Paul} and Pedersen, {Ole Birger} and Mehmet Coskun and Troelsen, {Jesper Thorvald}",
note = "This article is protected by copyright. All rights reserved.",
year = "2021",
doi = "10.1002/2211-5463.13161",
language = "English",
volume = "11",
pages = "1638--1644",
journal = "FEBS Open Bio",
issn = "2211-5463",
publisher = "FEBS Press",
number = "6",

}

RIS

TY - JOUR

T1 - CDX2 regulates interleukin-33 gene expression in intestinal epithelial cells (LS174T)

AU - Larsen, Sylvester

AU - Seidelin, Jakob Benedict

AU - Davidsen, Johanne

AU - Dahlgaard, Katja

AU - Nielsen, Claus Henrik

AU - Bennett, Eric Paul

AU - Pedersen, Ole Birger

AU - Coskun, Mehmet

AU - Troelsen, Jesper Thorvald

N1 - This article is protected by copyright. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Dysregulation of interleukin 33 (IL-33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the intestinal-specific transcription factor CDX2 is a key component. In this study, we investigated whether CDX2 regulates IL33 mRNA expression. We examined IL33 mRNA expression in primary colonic epithelial cells from healthy humans and epithelial cell lines, revealing high expression levels in primary colonic and LS174T cells. Combining genomics data (ChIP-Seq, RNA-Seq) and IL33 promoter analyses in LS174T cells revealed intronic enhancer activity in the IL33 gene that is dependent on CDX2 expression. Western blotting and qRT-PCR confirmed that IL33 expression is upregulated in a CDX2 concentration-dependent manner, thereby providing the first evidence that CDX2 regulates the expression of IL33.

AB - Dysregulation of interleukin 33 (IL-33) has been implicated in the pathogenesis of several autoimmune and inflammatory diseases, but few studies have examined transcriptional regulation of the IL33 gene. In the intestines, gene regulation is controlled by a transcription factor network of which the intestinal-specific transcription factor CDX2 is a key component. In this study, we investigated whether CDX2 regulates IL33 mRNA expression. We examined IL33 mRNA expression in primary colonic epithelial cells from healthy humans and epithelial cell lines, revealing high expression levels in primary colonic and LS174T cells. Combining genomics data (ChIP-Seq, RNA-Seq) and IL33 promoter analyses in LS174T cells revealed intronic enhancer activity in the IL33 gene that is dependent on CDX2 expression. Western blotting and qRT-PCR confirmed that IL33 expression is upregulated in a CDX2 concentration-dependent manner, thereby providing the first evidence that CDX2 regulates the expression of IL33.

U2 - 10.1002/2211-5463.13161

DO - 10.1002/2211-5463.13161

M3 - Journal article

C2 - 33838073

VL - 11

SP - 1638

EP - 1644

JO - FEBS Open Bio

JF - FEBS Open Bio

SN - 2211-5463

IS - 6

ER -

ID: 260306206