Parental Acceptance Rate of Testicular Tissue Cryopreservation in Danish Boys with Cryptorchidism
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Parental Acceptance Rate of Testicular Tissue Cryopreservation in Danish Boys with Cryptorchidism. / Hildorf, Simone; Cortes, Dina; Gül, Murat; Dong, Lihua; Kristensen, Stine G; Jensen, Christian F S; Clasen-Linde, Erik; Fedder, Jens; Andersen, Claus Y; Hoffmann, Eva R; Sønksen, Jens; Fossum, Magdalena; Thorup, Jørgen.
I: Sexual Development, Bind 13, Nr. 5-6, 2020, s. 246-257.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Parental Acceptance Rate of Testicular Tissue Cryopreservation in Danish Boys with Cryptorchidism
AU - Hildorf, Simone
AU - Cortes, Dina
AU - Gül, Murat
AU - Dong, Lihua
AU - Kristensen, Stine G
AU - Jensen, Christian F S
AU - Clasen-Linde, Erik
AU - Fedder, Jens
AU - Andersen, Claus Y
AU - Hoffmann, Eva R
AU - Sønksen, Jens
AU - Fossum, Magdalena
AU - Thorup, Jørgen
N1 - © 2020 S. Karger AG, Basel.
PY - 2020
Y1 - 2020
N2 - Despite orchidopexy within the first year of life, 20-25% of boys with nonsyndromic cryptorchidism may risk infertility according to histological and hormonal data obtained during surgery. The aim of this study was to evaluate the acceptance rate of testicular tissue cryopreservation among parents of prepubertal boys with cryptorchidism. Fourteen boys with cryptorchidism and high infertility risk were offered cryopreservation as an additional procedure after orchidopexy based on abnormal histopathological findings at primary surgery, whereas 27 boys with bilateral cryptorchidism were offered cryopreservation at the initial orchidopexy. A total of 90% of parents (37/41, 13/14, and 24/27) gave consent to perform cryopreservation, despite being well-informed that the procedural efficacy is largely unproven and may only be needed in about 20% of cases. The number of germ cells per tubule cross-section was 0.03-1.70 (median 0.37) and 22 boys (54%, 22/41) had a value below the lower range. Twelve boys (29%, 12/41) had no type A dark spermatogonia in their biopsy. Cryopreservation of testicular tissue is the first step to introduce spermatogonial stem cell-based therapy into clinical male infertility treatment. At the time of orchidopexy, a testicular biopsy can be collected to ascertain the infertility risk, and it may be an option for boys with bilateral cryptorchidism to have spermatogonial stem cells frozen as a fertility reserve.
AB - Despite orchidopexy within the first year of life, 20-25% of boys with nonsyndromic cryptorchidism may risk infertility according to histological and hormonal data obtained during surgery. The aim of this study was to evaluate the acceptance rate of testicular tissue cryopreservation among parents of prepubertal boys with cryptorchidism. Fourteen boys with cryptorchidism and high infertility risk were offered cryopreservation as an additional procedure after orchidopexy based on abnormal histopathological findings at primary surgery, whereas 27 boys with bilateral cryptorchidism were offered cryopreservation at the initial orchidopexy. A total of 90% of parents (37/41, 13/14, and 24/27) gave consent to perform cryopreservation, despite being well-informed that the procedural efficacy is largely unproven and may only be needed in about 20% of cases. The number of germ cells per tubule cross-section was 0.03-1.70 (median 0.37) and 22 boys (54%, 22/41) had a value below the lower range. Twelve boys (29%, 12/41) had no type A dark spermatogonia in their biopsy. Cryopreservation of testicular tissue is the first step to introduce spermatogonial stem cell-based therapy into clinical male infertility treatment. At the time of orchidopexy, a testicular biopsy can be collected to ascertain the infertility risk, and it may be an option for boys with bilateral cryptorchidism to have spermatogonial stem cells frozen as a fertility reserve.
U2 - 10.1159/000511158
DO - 10.1159/000511158
M3 - Journal article
C2 - 33080598
VL - 13
SP - 246
EP - 257
JO - Sexual Development
JF - Sexual Development
SN - 1661-5425
IS - 5-6
ER -
ID: 250352528