Downregulation of miR-125b in metastatic cutaneous malignant melanoma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Martin Glud
  • Maria Rossing
  • Christoffer Hother
  • Line Holst
  • Nina Hastrup
  • Nielsen, Finn Cilius
  • Robert Gniadecki
  • Krzysztof T Drzewiecki
This study aimed to identify microRNA species involved in the earliest metastatic event in cutaneous malignant melanoma (MM). Samples from 28 patients with MM [stage T2 (tumor), M0 (distant metastasis)] were grouped by the presence of micrometastasis in the sentinel lymph nodes (N0/N1). Melanoma cells were harvested from primary, cutaneous MM tumors by laser-capture microdissection, and microRNA expression profiles were obtained by the microarray technique. Results were validated by quantitative reverse transcription PCR. We found that miR-125b was downregulated in the primary cutaneous melanomas that produced early metastases (T2, N1, M0) compared with the sentinel lymph node-negative (T2, N0, M0) melanomas. MiR-125b has earlier been found to be downregulated in other tumor types and in atypic naevi compared with the common acquired naevi. In conclusion, miR-125b may be involved in an early progression of cutaneous MM.
TidsskriftMelanoma Research
Udgave nummer6
Sider (fra-til)479-84
Antal sider6
StatusUdgivet - 1 dec. 2010

ID: 34073581