Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1

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Standard

Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1. / Poon, Anna Fong-Yee; Li, Tong; Pires, Carlota; Nielsen, Troels Tolstrup; Nielsen, Jørgen Erik; Holst, Bjørn; Dinnyes, Andras; Hyttel, Poul; Freude, Kristine.

I: Stem Cell Research, Bind 17, Nr. 3, 11.2016, s. 470-473.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Poon, AF-Y, Li, T, Pires, C, Nielsen, TT, Nielsen, JE, Holst, B, Dinnyes, A, Hyttel, P & Freude, K 2016, 'Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1', Stem Cell Research, bind 17, nr. 3, s. 470-473. https://doi.org/10.1016/j.scr.2016.09.016

APA

Poon, A. F-Y., Li, T., Pires, C., Nielsen, T. T., Nielsen, J. E., Holst, B., Dinnyes, A., Hyttel, P., & Freude, K. (2016). Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1. Stem Cell Research, 17(3), 470-473. https://doi.org/10.1016/j.scr.2016.09.016

Vancouver

Poon AF-Y, Li T, Pires C, Nielsen TT, Nielsen JE, Holst B o.a. Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1. Stem Cell Research. 2016 nov.;17(3):470-473. https://doi.org/10.1016/j.scr.2016.09.016

Author

Poon, Anna Fong-Yee ; Li, Tong ; Pires, Carlota ; Nielsen, Troels Tolstrup ; Nielsen, Jørgen Erik ; Holst, Bjørn ; Dinnyes, Andras ; Hyttel, Poul ; Freude, Kristine. / Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1. I: Stem Cell Research. 2016 ; Bind 17, Nr. 3. s. 470-473.

Bibtex

@article{3f38112e94594fadabb8aa1ae31c40c7,
title = "Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1",
abstract = "Mutations in presenilin 1 (PSEN1) lead to the most aggressive form of familial Alzheimer's disease (AD). Human induced pluripotent stem cells (hiPSCs) derived from AD patients can be differentiated and used for disease modeling. Here, we derived hiPSC from skin fibroblasts obtained from an AD patient carrying a L282F mutation in PSEN1. We transfected skin fibroblasts with episomal iPSC reprogramming vectors targeting human OCT4, SOX2, L-MYC, KLF4, NANOG, LIN28, and short hairpin RNA against TP53. Our hiPSC line, L282F-hiPSC, displayed typical stem cell characteristics with consistent expression of pluripotency genes and the ability to differentiation into the three germ layers.",
author = "Poon, {Anna Fong-Yee} and Tong Li and Carlota Pires and Nielsen, {Troels Tolstrup} and Nielsen, {J{\o}rgen Erik} and Bj{\o}rn Holst and Andras Dinnyes and Poul Hyttel and Kristine Freude",
note = "Copyright {\textcopyright} 2016. Published by Elsevier B.V.",
year = "2016",
month = nov,
doi = "10.1016/j.scr.2016.09.016",
language = "English",
volume = "17",
pages = "470--473",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Derivation of induced pluripotent stem cells from a familial Alzheimer's disease patient carrying the L282F mutation in presenilin 1

AU - Poon, Anna Fong-Yee

AU - Li, Tong

AU - Pires, Carlota

AU - Nielsen, Troels Tolstrup

AU - Nielsen, Jørgen Erik

AU - Holst, Bjørn

AU - Dinnyes, Andras

AU - Hyttel, Poul

AU - Freude, Kristine

N1 - Copyright © 2016. Published by Elsevier B.V.

PY - 2016/11

Y1 - 2016/11

N2 - Mutations in presenilin 1 (PSEN1) lead to the most aggressive form of familial Alzheimer's disease (AD). Human induced pluripotent stem cells (hiPSCs) derived from AD patients can be differentiated and used for disease modeling. Here, we derived hiPSC from skin fibroblasts obtained from an AD patient carrying a L282F mutation in PSEN1. We transfected skin fibroblasts with episomal iPSC reprogramming vectors targeting human OCT4, SOX2, L-MYC, KLF4, NANOG, LIN28, and short hairpin RNA against TP53. Our hiPSC line, L282F-hiPSC, displayed typical stem cell characteristics with consistent expression of pluripotency genes and the ability to differentiation into the three germ layers.

AB - Mutations in presenilin 1 (PSEN1) lead to the most aggressive form of familial Alzheimer's disease (AD). Human induced pluripotent stem cells (hiPSCs) derived from AD patients can be differentiated and used for disease modeling. Here, we derived hiPSC from skin fibroblasts obtained from an AD patient carrying a L282F mutation in PSEN1. We transfected skin fibroblasts with episomal iPSC reprogramming vectors targeting human OCT4, SOX2, L-MYC, KLF4, NANOG, LIN28, and short hairpin RNA against TP53. Our hiPSC line, L282F-hiPSC, displayed typical stem cell characteristics with consistent expression of pluripotency genes and the ability to differentiation into the three germ layers.

U2 - 10.1016/j.scr.2016.09.016

DO - 10.1016/j.scr.2016.09.016

M3 - Journal article

C2 - 27789396

VL - 17

SP - 470

EP - 473

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 3

ER -

ID: 172816808