Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1

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Standard

Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1. / Poon, Anna Fong-Yee; Schmid, Benjamin; Pires, Carlota; Nielsen, Troels Tolstrup; Hjermind, Lena Elisabeth; Nielsen, Jørgen Erik; Holst, Bjørn; Hyttel, Poul; Freude, Kristine.

I: Stem Cell Research, Bind 17, Nr. 3, 11.2016, s. 466-469.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Poon, AF-Y, Schmid, B, Pires, C, Nielsen, TT, Hjermind, LE, Nielsen, JE, Holst, B, Hyttel, P & Freude, K 2016, 'Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1', Stem Cell Research, bind 17, nr. 3, s. 466-469. https://doi.org/10.1016/j.scr.2016.09.018

APA

Poon, A. F-Y., Schmid, B., Pires, C., Nielsen, T. T., Hjermind, L. E., Nielsen, J. E., Holst, B., Hyttel, P., & Freude, K. (2016). Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1. Stem Cell Research, 17(3), 466-469. https://doi.org/10.1016/j.scr.2016.09.018

Vancouver

Poon AF-Y, Schmid B, Pires C, Nielsen TT, Hjermind LE, Nielsen JE o.a. Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1. Stem Cell Research. 2016 nov.;17(3):466-469. https://doi.org/10.1016/j.scr.2016.09.018

Author

Poon, Anna Fong-Yee ; Schmid, Benjamin ; Pires, Carlota ; Nielsen, Troels Tolstrup ; Hjermind, Lena Elisabeth ; Nielsen, Jørgen Erik ; Holst, Bjørn ; Hyttel, Poul ; Freude, Kristine. / Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1. I: Stem Cell Research. 2016 ; Bind 17, Nr. 3. s. 466-469.

Bibtex

@article{1e7f9709c1474d4eae5c855fb7e746ca,
title = "Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1",
abstract = "Mutations in the presenilin 1 (PSEN1) gene lead to the most aggressive form of familial Alzheimer's disease (AD). Human induced pluripotent stem cells (hiPSCs) derived from AD patients and subsequently differentiated can be used for disease modeling. We have previously generated a hiPSC line from a familial AD patient carrying a L150P point mutation in PSEN1. Here we used CRISPR/Cas9 gene editing to correct for the single base pair mutation. This gene-corrected line, L150P-GC-hiPSC, serves as an isogenic control to the mutant line for future investigation of mechanisms and cellular phenotypes altered by this specific PSEN1 mutation.",
author = "Poon, {Anna Fong-Yee} and Benjamin Schmid and Carlota Pires and Nielsen, {Troels Tolstrup} and Hjermind, {Lena Elisabeth} and Nielsen, {J{\o}rgen Erik} and Bj{\o}rn Holst and Poul Hyttel and Kristine Freude",
note = "Copyright {\textcopyright} 2016 The Authors. Published by Elsevier B.V. All rights reserved.",
year = "2016",
month = nov,
doi = "10.1016/j.scr.2016.09.018",
language = "English",
volume = "17",
pages = "466--469",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Generation of a gene-corrected isogenic control hiPSC line derived from a familial Alzheimer's disease patient carrying a L150P mutation in presenilin 1

AU - Poon, Anna Fong-Yee

AU - Schmid, Benjamin

AU - Pires, Carlota

AU - Nielsen, Troels Tolstrup

AU - Hjermind, Lena Elisabeth

AU - Nielsen, Jørgen Erik

AU - Holst, Bjørn

AU - Hyttel, Poul

AU - Freude, Kristine

N1 - Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

PY - 2016/11

Y1 - 2016/11

N2 - Mutations in the presenilin 1 (PSEN1) gene lead to the most aggressive form of familial Alzheimer's disease (AD). Human induced pluripotent stem cells (hiPSCs) derived from AD patients and subsequently differentiated can be used for disease modeling. We have previously generated a hiPSC line from a familial AD patient carrying a L150P point mutation in PSEN1. Here we used CRISPR/Cas9 gene editing to correct for the single base pair mutation. This gene-corrected line, L150P-GC-hiPSC, serves as an isogenic control to the mutant line for future investigation of mechanisms and cellular phenotypes altered by this specific PSEN1 mutation.

AB - Mutations in the presenilin 1 (PSEN1) gene lead to the most aggressive form of familial Alzheimer's disease (AD). Human induced pluripotent stem cells (hiPSCs) derived from AD patients and subsequently differentiated can be used for disease modeling. We have previously generated a hiPSC line from a familial AD patient carrying a L150P point mutation in PSEN1. Here we used CRISPR/Cas9 gene editing to correct for the single base pair mutation. This gene-corrected line, L150P-GC-hiPSC, serves as an isogenic control to the mutant line for future investigation of mechanisms and cellular phenotypes altered by this specific PSEN1 mutation.

U2 - 10.1016/j.scr.2016.09.018

DO - 10.1016/j.scr.2016.09.018

M3 - Journal article

C2 - 27789395

VL - 17

SP - 466

EP - 469

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 3

ER -

ID: 172816879