Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1

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Standard

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1. / Li, Tong; Pires, Carlota; Nielsen, Troels Tolstrup; Waldemar, Gunhild; Hjermind, Lena E.; Nielsen, Jørgen E.; Dinnyes, Andras; Hyttel, Poul; Freude, Kristine K.

I: Stem Cell Research, Bind 16, Nr. 2, 03.2016, s. 229-332.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Li, T, Pires, C, Nielsen, TT, Waldemar, G, Hjermind, LE, Nielsen, JE, Dinnyes, A, Hyttel, P & Freude, KK 2016, 'Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1', Stem Cell Research, bind 16, nr. 2, s. 229-332. https://doi.org/10.1016/j.scr.2016.01.002

APA

Li, T., Pires, C., Nielsen, T. T., Waldemar, G., Hjermind, L. E., Nielsen, J. E., Dinnyes, A., Hyttel, P., & Freude, K. K. (2016). Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1. Stem Cell Research, 16(2), 229-332. https://doi.org/10.1016/j.scr.2016.01.002

Vancouver

Li T, Pires C, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE o.a. Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1. Stem Cell Research. 2016 mar.;16(2):229-332. https://doi.org/10.1016/j.scr.2016.01.002

Author

Li, Tong ; Pires, Carlota ; Nielsen, Troels Tolstrup ; Waldemar, Gunhild ; Hjermind, Lena E. ; Nielsen, Jørgen E. ; Dinnyes, Andras ; Hyttel, Poul ; Freude, Kristine K. / Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1. I: Stem Cell Research. 2016 ; Bind 16, Nr. 2. s. 229-332.

Bibtex

@article{700d94f744a846c2be60d076b6790a4b,

title = "Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1",

abstract = "Skin fibroblasts were obtained from a 48-year-old presymptomatic woman carrying a A79V mutation in the presenilin 1 gene (PSEN1), causing Alzheimer's disease (AD). Induced pluripotent stem cell (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. A79V-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported A79V-iPSCs line may be a useful resource for in vitro modeling of familial AD.",

keywords = "Alzheimer Disease, Base Sequence, Cell Differentiation, Cells, Cultured, Cellular Reprogramming, Exons, Female, Fibroblasts, Humans, Induced Pluripotent Stem Cells, Karyotype, Microscopy, Fluorescence, Middle Aged, Polymorphism, Single Nucleotide, Presenilin-1, Real-Time Polymerase Chain Reaction, Transcription Factors, Journal Article, Research Support, Non-U.S. Gov't",

author = "Tong Li and Carlota Pires and Nielsen, {Troels Tolstrup} and Gunhild Waldemar and Hjermind, {Lena E.} and Nielsen, {J{\o}rgen E.} and Andras Dinnyes and Poul Hyttel and Freude, {Kristine K.}",

year = "2016",

month = mar,

doi = "10.1016/j.scr.2016.01.002",

language = "English",

volume = "16",

pages = "229--332",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

number = "2",

}

RIS

TY - JOUR

T1 - Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying an A79V mutation in PSEN1

AU - Li, Tong

AU - Pires, Carlota

AU - Nielsen, Troels Tolstrup

AU - Waldemar, Gunhild

AU - Hjermind, Lena E.

AU - Nielsen, Jørgen E.

AU - Dinnyes, Andras

AU - Hyttel, Poul

AU - Freude, Kristine K.

PY - 2016/3

Y1 - 2016/3

N2 - Skin fibroblasts were obtained from a 48-year-old presymptomatic woman carrying a A79V mutation in the presenilin 1 gene (PSEN1), causing Alzheimer's disease (AD). Induced pluripotent stem cell (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. A79V-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported A79V-iPSCs line may be a useful resource for in vitro modeling of familial AD.

AB - Skin fibroblasts were obtained from a 48-year-old presymptomatic woman carrying a A79V mutation in the presenilin 1 gene (PSEN1), causing Alzheimer's disease (AD). Induced pluripotent stem cell (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. A79V-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported A79V-iPSCs line may be a useful resource for in vitro modeling of familial AD.

KW - Alzheimer Disease

KW - Base Sequence

KW - Cell Differentiation

KW - Cells, Cultured

KW - Cellular Reprogramming

KW - Exons

KW - Female

KW - Fibroblasts

KW - Humans

KW - Induced Pluripotent Stem Cells

KW - Karyotype

KW - Microscopy, Fluorescence

KW - Middle Aged

KW - Polymorphism, Single Nucleotide

KW - Presenilin-1

KW - Real-Time Polymerase Chain Reaction

KW - Transcription Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.scr.2016.01.002

DO - 10.1016/j.scr.2016.01.002

M3 - Journal article

C2 - 27345973

VL - 16

SP - 229

EP - 332

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 2

ER -

ID: 173167997

Institut for Klinisk Medicin