Neuroprotective and anti-inflammatory mechanisms are activated early in optic neuritis
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Neuroprotective and anti-inflammatory mechanisms are activated early in optic neuritis. / Tsakiri, A; Ravanidis, S; Lagoudaki, R; Poulatsidou, K-N; Svane, I M; Frederiksen, Jette Lautrup; Grigoriadis, N.
I: Acta Neurologica Scandinavica, Bind 131, Nr. 5, 05.2015, s. 305-12.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Neuroprotective and anti-inflammatory mechanisms are activated early in optic neuritis
AU - Tsakiri, A
AU - Ravanidis, S
AU - Lagoudaki, R
AU - Poulatsidou, K-N
AU - Svane, I M
AU - Frederiksen, Jette Lautrup
AU - Grigoriadis, N
N1 - © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2015/5
Y1 - 2015/5
N2 - OBJECTIVE: The aim of the study was to investigate the expression of different immunological mediators in blood and CSF in patients with acute ON and to estimate whether they were implicated in pro- or anti-inflammatory or even regulatory reactions in comparison with a healthy control group (HC).METHODS: Sixty-four patients between 18 and 59 years of age suffering by acute ON, onset of <4 weeks, were included in the study. Visual tests and brain magnetic resonance imaging (MRI) were performed in ON. Blood and CSF samples were collected from untreated patients and from a gender- and age-matched voluntary HC (n = 32). The mRNA expression of distinct cytokines and neurotrophic factors was assessed by semi/quantitative real-time PCR (RT-PCR).RESULTS: Brain- and glial cell-derived neurotrophic factor (BDNF and GDNF) and interleukin 10 (IL-10) expression was significantly increased in the CSF compared to the blood in both ON and HC (P < 0.001). In the CSF increased levels of BDNF and GDNF of the ON group were positively correlated with the presence of oligoclonal bands (OB). Additionally, patients with gadolinium (gd+) lesions on brain MRI showed increased levels of IL-5 in blood (P = 0.03).CONCLUSION: Our data indicate that both immuno-regulatory and neuroprotective mechanisms may potentially take place relatively early in the course of the ON. The presence of neurotrophic factors in healthy CSF and their overexpression already during the acute phase of ON supports the alertness of CNS defence mechanisms ready to be activated during degenerative events, such as destruction of the myelin.
AB - OBJECTIVE: The aim of the study was to investigate the expression of different immunological mediators in blood and CSF in patients with acute ON and to estimate whether they were implicated in pro- or anti-inflammatory or even regulatory reactions in comparison with a healthy control group (HC).METHODS: Sixty-four patients between 18 and 59 years of age suffering by acute ON, onset of <4 weeks, were included in the study. Visual tests and brain magnetic resonance imaging (MRI) were performed in ON. Blood and CSF samples were collected from untreated patients and from a gender- and age-matched voluntary HC (n = 32). The mRNA expression of distinct cytokines and neurotrophic factors was assessed by semi/quantitative real-time PCR (RT-PCR).RESULTS: Brain- and glial cell-derived neurotrophic factor (BDNF and GDNF) and interleukin 10 (IL-10) expression was significantly increased in the CSF compared to the blood in both ON and HC (P < 0.001). In the CSF increased levels of BDNF and GDNF of the ON group were positively correlated with the presence of oligoclonal bands (OB). Additionally, patients with gadolinium (gd+) lesions on brain MRI showed increased levels of IL-5 in blood (P = 0.03).CONCLUSION: Our data indicate that both immuno-regulatory and neuroprotective mechanisms may potentially take place relatively early in the course of the ON. The presence of neurotrophic factors in healthy CSF and their overexpression already during the acute phase of ON supports the alertness of CNS defence mechanisms ready to be activated during degenerative events, such as destruction of the myelin.
KW - Adolescent
KW - Adult
KW - Female
KW - Humans
KW - Inflammation Mediators
KW - Interleukin-10
KW - Magnetic Resonance Imaging
KW - Male
KW - Middle Aged
KW - Nerve Growth Factors
KW - Oligoclonal Bands
KW - Optic Neuritis
KW - Real-Time Polymerase Chain Reaction
KW - Young Adult
U2 - 10.1111/ane.12344
DO - 10.1111/ane.12344
M3 - Journal article
C2 - 25565254
VL - 131
SP - 305
EP - 312
JO - Acta Neurologica Scandinavica
JF - Acta Neurologica Scandinavica
SN - 0001-6314
IS - 5
ER -
ID: 161585849